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    Shady Adnan Awad

    Shady Adnan Awad

    1Hematology Research Constituent Helsinki, Lincoln of Helsingfors and Helsingfors University Sickbay Comprehensive Crab Center, Port, Finland

    2Translational Immunology Research Info and Arm of Clinical Chemistry keep from Hematology, Institution of higher education of Helsingfors, Helsinki, Finland

    3Foundation for say publicly Finnish Human Institute, Port, Finland

    4Clinical Pathology Department, Countrywide Cancer Alliance, Cairo College, Cairo, Egypt

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    1,2,3,4,11,∗, Olli Dufva

    Olli Dufva

    1Hematology Research Residential home Helsinki, Academia of Port and Helsingfors University Health centre Comprehensive Someone Center, Helsingfors, Finland

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    1,2,5, Jurist Klievink

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    1Hematology Research Lodging Helsinki, College of Port and Helsingfors University Dispensary Comprehensive Mortal Center, Port, Finland

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    Patient-derived response estimates from zero-passage organoids of luminal breast cancer

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    Breast Cancer Researchvolume 26, Article number:  () Cite this article

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    Abstract

    Background

    Primary luminal breast cancer cells lose their identity rapidly in standard tissue culture, which is problematic for testing hormone interventions and molecular pathways specific to the luminal subtype. Breast cancer organoids are thought to retain tumor characteristics better, but long-term viability of luminal-subtype cases is a persistent challenge. Our goal was to adapt short-term organoids of luminal breast cancer for parallel testing of genetic and pharmacologic perturbations.

    Methods

    We freshly isolated patient-derived cells from luminal tumor scrapes, miniaturized the organoid format into 5 µl replicates for increased throughput, and set an endpoint of 14 days to minimize drift. Therapeutic hormone targeting was mimicked in these “zero–passage” organoids by withdrawing β-estradiol and adding 4-hydroxytamoxifen. We also examined sulforaphane as an electrophilic stress and commercial nutraceutical with reported anti-cancer properties. Downstream mechanisms were tested genetically by lentiviral